Part:BBa_K1506001:Design
Superfolder GFP with only common codons
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 58
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This GFP codes for the same green florescent protein as superfolder GFP, but it is comprised only of common codons. Previous researchers have determined through a statistical analysis of the entire genome of E. coli that some degenerate codons occur more often in protein coding sequences and some are more infrequent. These are referred to as common and rare codons. The hypothesis that common codons will lead to higher expression of proteins is based on the idea that cellular DNA has become optimized through evolution to efficiently translate proteins necessary to their survival. For each amino acid coded by this GFP, the most commonly occurring codon in the genome of E. coli that specifies that amino acid was used, thus creating a coding sequence of only common codons.
Source
This part is entirely synthetic.